pQUAST>stop>mCD8-GFP

LOCUS       pQUAST>stop>mCD8-GFP               12835 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pQUAST>stop>mCD8-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
  TITLE     The Q System: A Repressible Binary System for Transgene Expression, 
            Lineage Tracing, and Mosaic Analysis
  JOURNAL   Volume 141, Issue 3, 536-548, 30 April 2010
  PUBMED    20434990
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pQUAST>stop>mCD8-GFP|
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(994..1854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    2761..2993
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    3502..3549
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          3669..3734
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3864..3884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4156..4290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5112..5159
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5204..5869
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             5876..6592
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5906..5934)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6529..6549
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          6676..6741
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6871..6891
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7163..7297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          7464..7529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7659..7679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7951..8085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8112..8130)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8590..8661,9056..9329,9404..10058,10120..10435,
                     10656..10787,10858..11472)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    12250..12835
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgagggtac ccggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat
     3541 aggaacttca gagcgctttt gaagctagag aattcgcgca ctagtgctag aggatctttg
     3601 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     3661 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     3721 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     3781 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     3841 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     3901 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     3961 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     4021 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     4081 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4141 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4261 ctcatcaatg tatcttatca tgtctggatc tgcgtcgcgc cacttcaacg ctcgatggga
     4321 gcgtcattgg tgggcggggt aaccgtcgaa atcagtgttt acgcttccaa tcgcaacaaa
     4381 aaattcactg caacactgaa aagcatacga aaacgatgaa gattgtacga gaaaccataa
     4441 agtattttat ccacaaagac acgtatagca gaaaagccaa gttaactcgg cgataagttg
     4501 tgtacacaag aataaaatcg gccagattca gtgttgtcag aaataagaaa accccactat
     4561 gtttttcttt gccttttctt tctcccagcg atcattcatt tcgtggtgaa agaacggggt
     4621 cattgcacgg agtttcgact gcgggaaagc agagctgccg ttcacttcgt ctataattag
     4681 cgctttctat tttccccgat tcgggccgct gctgcgcttt tccgcctgct gtttgtggca
     4741 agtgtagcag caggctgtgc acgcagtgtg gcatgcactt ggctttccac cgttggtatc
     4801 gattctctgg gacgatgagt cattcctttc ggggccacag cataatcgtt gccagctcac
     4861 cgaaatggtg acttcatttc ttaactgccg tcaagcatgc gattgtacat acatacatat
     4921 ttatatatgt acatatttat gtgactatgg taggtcgata taatagcaat caacgcaagc
     4981 aaatgtgtca gtcctgctta caggaacgat tctatttagt aattttcgtt gtataaagta
     5041 attatgtatg tatgtaagcc ccataaatct gaaacaatta ggcaaaacca tgcgaggatc
     5101 cgggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
     5161 gagcgctttt gaagctgggt accgcggccg cggctcgagc aaaatggcct caccgttgac
     5221 ccgctttctg tcgctgaacc tgctgctgct gggtgagtcg attatcctgg ggagtggaga
     5281 agctaagcca caggcacccg aactccgaat ctttccaaag aaaatggacg ccgaacttgg
     5341 tcagaaggtg gacctggtat gtgaagtgtt ggggtccgtt tcgcaaggat gctcttggct
     5401 cttccagaac tccagctcca aactccccca gcccaccttc gttgtctata tggcttcatc
     5461 ccacaacaag ataacgtggg acgagaagct gaattcgtcg aaactgtttt ctgccatgag
     5521 ggacacgaat aataagtacg ttctcaccct gaacaagttc agcaaggaaa acgaaggcta
     5581 ctatttctgc tcagtcatca gcaactcggt gatgtacttc agttctgtcg tgccagtcct
     5641 tcagaaagtg aactctacta ctaccaagcc agtgctgcga actccctcac ctgtgcaccc
     5701 taccgggaca tctcagcccc agagaccaga agattgtcgg ccccgtggct cagtgaaggg
     5761 gaccggattg gacttcgcct gtgatattta catctgggca cccttggccg gaatctgcgt
     5821 ggcccttctg ctgtccttga tcatcactct catctgctac cacagccgcg gatccatgag
     5881 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     5941 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     6001 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     6061 tactttaact tatggtgttc aatgcttttc aagataccca gatcatatga aacagcatga
     6121 ctttttcaag agtgccatgc ccgaaggtta tgtccaggaa agaactatat ttttcaaaga
     6181 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     6241 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     6301 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     6361 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     6421 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     6481 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     6541 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aatctagagg
     6601 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     6661 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     6721 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     6781 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     6841 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     6901 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     6961 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     7021 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     7081 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     7141 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     7201 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7261 gtccaaactc atcaatgtat cttatcatgt ctggatccac tagaaggcct tagtatgggg
     7321 taccccaagg gcgaattctg cagatatcca tcacactggc ggccgcggct cgagggtacc
     7381 tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     7441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     7501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     7561 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     7621 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     7681 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     7741 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     7801 agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     7861 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     7921 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     7981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcggatc cactagaagg
     8101 ccttagtatg tatgtaagtt aataaaaccc ttttttggag aatgtagatt taaaaaaaca
     8161 tatttttttt ttatttttta ctgcactgga catcattgaa cttatctgat cagttttaaa
     8221 tttacttcga tccaagggta tttgaagtac caggttcttt cgattacctc tcactcaaaa
     8281 tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg
     8341 tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acctagcgtc
     8401 aatgtccgcc ttcagttgca ctttgtcagc ggtttcgtga cgaagctcca agcggtttac
     8461 gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt
     8521 tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa
     8581 atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca
     8641 tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat
     8701 ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa
     8761 aatcccaaac cgcaatcgca caaacaaata gtgacacgaa acagattatt ctggtagctg
     8821 tgctcgctat ataagacaat ttttaagatc atatcatgat caagacatct aaaggcattc
     8881 attttcgact acattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct
     8941 agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc gttttgttcg
     9001 gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga
     9061 cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg
     9121 cacgctcctg ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga
     9181 gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc
     9241 cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc
     9301 ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt
     9361 gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat
     9421 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
     9481 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
     9541 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
     9601 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaggc catggtgcgg
     9661 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
     9721 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
     9781 tccggcggag aaaggaagcg tctggcattc gcctccgagg cactaaccga tccgccgctt
     9841 ctgatctgcg atgagcccac ctccggactg gactcattta ccgcccacag cgtcgtccag
     9901 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
     9961 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
    10021 ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa
    10081 gggtatctag cattacatta catctcaact cctatccagc gtgggtgccc agtgtcctac
    10141 caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat
    10201 cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gctattagca aagtagcccg
    10261 ggatatggag cagttgttgg ccaccaaaaa tttggagaag ccactggagc agccggagaa
    10321 tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg
    10381 gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag
    10441 tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat
    10501 tttagttaga attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt
    10561 tatagttttg cagaaaataa ataaatttca tttaactcgc gaacatgttg aagatatgaa
    10621 tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca
    10681 tctttttggg ccaacaactc acgcaagtgg gcgtgatgaa tatcaacgga gccatcttcc
    10741 tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta agtcttgttt
    10801 agaatacatt tgcatattaa taatttacta actttctaat gaatcgattc gatttaggtg
    10861 ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac
    10921 acatactttc tgggcaaaac gattgccgaa ttaccgcttt ttctcacagt gccactggtc
    10981 ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac
    11041 tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc
    11101 tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc
    11161 ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
    11221 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
    11281 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
    11341 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
    11401 gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga
    11461 cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt accattatta
    11521 ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat
    11581 aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag
    11641 actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact
    11701 cgtttatcgc tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg
    11761 cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc
    11821 ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta
    11881 gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa
    11941 ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc
    12001 tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct
    12061 gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt
    12121 gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa
    12181 caactataat aataagaata catttaattt agaaaatgct tggatttcac tggaactaga
    12241 attaattcgg ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc
    12301 tcaagctctt attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt
    12361 acataaatga ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag
    12421 ccttctttta tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg
    12481 agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcatta
    12541 aagtgggtat cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac
    12601 aattttctct taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg
    12661 cagcaaaatt tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa
    12721 aaaaattcgt ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg
    12781 tatacttcgg taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatg
//